Genetics of autoimmune diseases (systemic lupus erythematosus) in human.
Transmission - Disequilibrium Test as a tool to detect linkage (association) between complex disease and a genetic markers.
 slide 01 My Education: MS in Chemistry, Russia (1987). PhD in Molecular Biology (1996), The Netherlands. Post doctoral projects - UC Davis (1996-2000). |
 slide 02 PhD project in the field of Plant-Microbe interaction. Wageningen Agricultural University, The Netherlands, Dr. Ton Bisseling's lab (1991-1996). |
 slide 03 Host specific interaction between Pea and rhizobia (topic of PhD Thesis 1991-1996). |
 slide 04 First Post doctoral project: Telomere - binding protein(s) in bull sperm. UC Davis, School of Medicine (1996-1999). |
 slide 05 Second Post doctoral project: Genetics of autoimmune diseases in human. UC Davis, Dr. Mike Seldin's lab (1999-2000). |
 slide 06 Systemic lupus erythematosus (SLE). |
 slide 07 Genes which were involved into genotyping of SLE. |
 slide 08 Transmission - Disequilibrium Test as a tool to detect linkage (association) between complex disease and a genetic markers. |
 slide 09 Trios, preferential transmission of specific allele(s) from parents to to affected child. |
 slide 10 Principles of Genotyping using SNPs. |
 slide 11 Strategy of Genotyping. |
 slide 12 Sample handling and dataflow (plate format). |
 slide 13 Sample handling and dataflow (devices). |
 slide 14 Low cost high throughput genotyping and sequencing. |
 slide 15 Multiplexing of different markers. |
 slide 16 Single base extension. |
 slide 17 "Minus One" Method. |
 slide 18 Example of GeneScan file (paradise). |
 slide 19 Example of GeneScan file (hell). |
 slide 20 Example of GeneScan file (real life). |
 slide 21 Example of GeneScan file (allele dropout, insertion/deletion, real life). |
 slide 22 Example of GeneScan file (allele dropout, repeats, real life). |
 slide 23 Principles of SNPs searching and validation. |
 slide 24 Example of alignment of DNA sequences analyzed by Phred, Phrap and PolyPhred and viewed in Consed. |
 slide 25 Examples of chromatograms, SNP validation forward direction. |
 slide 26 Examples of chromatograms, SNP validation reverse direction. |
 slide 27 Examples of chromatograms, SNP validation two different alleles, single nucleotide insertion-deletion. |
 slide 28 Examples of chromatograms, SNP validation Single nucleotide insertion-deletion (heterozygote). |
 slide 29 Genotyping, example of separation of two alleles with single nucleotide insertion-deletion. |
 slide 30 Example of fake SNP. |
 slide 31 Examples of chromatograms of fake SNP. |
 slide 32 Data management and data flow. |
 slide 33 Screenshot of mySQL database server with PHP support (table setup). |
 slide 34 Screenshot of mySQL database server with PHP support (data entry). |
 slide 35 Screenshot of mySQL database server with PHP support (data management). |
 slide 36 Hardware and software environment. |
