Intro
step_01
step_02
step_03
step_04
step_05
step_06
step_07
step_08
step_09
step_10
step_11
step_12
step_13
step_14
step_15
step_16
step_17
step_18
step_19
step_20
step_21
step_22
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step_24
Credits
CGPDB
UC Davis
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Step 20: Usage of "Contig_Target" perl script and Primer3 program
In UNIX shell following command has been executed. By "-x" option we specify file name
with "target" info; "-a" option specifies tab-delimited file with consensus sequences.
$ perl contig_target_May_01_2003.pl -x primer_picker.in
-a tomato_ABC.fasta.cap.contigs.one_line.tab
Output file "primer_picker.in.out" will be
generated with detailed information about oligos (if found) for PCR. [Note, that
for contig 1104 primers were not designed by Primer3 program. Manual selection in this case,
probably, required].
Finally, set up and enjoy PCR:
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